Micro lab final- all unknown bacteria study play gram negative fermenters-citrobacter freudii-proteus vulgaris-proteus mirabilis-serratia marcescens-morganella morganii-klebsiella pneumoniae-enterobacter aerogenes-escherichia coli citrobacter freudii microbiology lab final identification of unknown bacteria flow chart 49 terms. Serratia marcescens has a long history in the church, as well as in microbiologynumerous historical incidents recount that the blood red pigment produced by s marcescens growing in bread was interpreted as a sign of bloods marcescens also grows well in damp basements, on food stored in damp places, and is a frequent contaminant in the laboratory. In this lab you will be innoculating plates and observing bacterial growth microscopes can then be used to identify specific bacteria this lab may take several days, keep all data and observations in a separate notebook to be compiled and organized into a final lab report.
• serratia marcescens is a motile, short rod-shaped, gram-negative, facultative anaerobe bacterium, classified as an opportunistic pathogen it was discovered in 1819 by bartolomeo bizio in padua, italy. Relationship to class instruction: biol&260 lab report due on: _____ material and methods cultures on bhi medium or tsa gram + bacillus cereus gram serratia marcescens. Lab report on unknown bacteria serratia marcescens in bacteria , temperature, ph, and other chemical agents all affect the expression of genes in this lab , the effect of temperature change on the gene which codes for a red pigment called prodigiosin of bacterium serratia marcescens is being tested. Appendix b unknown lab report guidelines 112 cain et al 3 revised spring 2013 tutorial website for wear lab coats (preferred) or aprons to protect clothes d) clean lab table with an antiseptic wash serratia marcescens streptococcus staphylococcus epidermidis staphylococcus intermedius.
Microbiology 20 biochemical unknown – spring 2009 (due may 14th) you should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on thursday may 14thout of a possible 70 points you. Microbiology unknown lab report kateryna petrakova introduction microorganisms cause a great amount of diseases for healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, therefore, to find an appropriate treatment. Ultra-violet radiations prevent the production of red pigment, prodigiosin in serratia marcescens by inducing mutation in its dna hypothesis maybe the disappearance of red color in serratia marcescens is caused by the uv light which causes mutations in its dna prediction if uv light mutates the. The identification of bacteria #1 was very easy since there are few organisms which have a red growth, and serratia marcescens is the only one that is on our unknowns list when the gram stain was inconclusive for this bacterium, it was sensible to use other tests to positively identify it. Serratia marcescens, a gram-negative, rod-shaped, facultative anaerobe (fig 1), is ubiquitous in water, soil, and natural settings it is easily grown in the lab and may serve as an ideal model for adaptation studies because of the natural color variation of s marcescens (gillen 2008.
We will write a custom essay sample on unknown lab report microbiology specifically for you for only $1638 $139/page order now serratia marcescens & bacillus cereus report microbiology laboratory report lab report microbiology identifying bacteria send me this sample. The sulfur reduction test is useful in differentiating enteric organisms the indole test is a component of the imvic series of tests, which is used for differentiating the enterobacteriaceaethe motility test is useful for testing a wide variety of organisms. Serratia species are opportunistic gram-negative bacteria classified in the tribe klebsielleae and the large family enterobacteriaceae serratia are widespread in the environment, but are not a common component of the human fecal flora.
How to write an unknown lab report in microbiology general unknown reports in microbiology are written in scientific format scientific writing is written escherichia coli serratia marcescens klebsiella oxytoca morganella morganii klebsiella pneumoniae pseudomonas aeruginosa pseudomonas aureofaciens indole test ( positive. In the lab experiment today, we want to find the effects of uv irradiation on two species of bacteria ( serratia marcescens , a gram negative rod and staphylococcus aureus , a gram positive coccus) with and without different spf sunscreens. Lab exercise 5: pure culture techniques 1 mixed culture of serratia marcescens, escherichia coli, and chromobacterium violaceum 1 environmental body broth (from lab exercise 4) 2 nutrient agar plates inoculating loop protocol: 1 prepare lab bench by removing extraneous items and cleaning surface with table disinfectant 2 label the bottom. Abstract this lab was done on mutations, specifically mutations on specific bacteria called serratia marcescens mutations are a very important part of biology understanding today.
Serratia marcescens an aerobic gram-negative bacillus that grows readily on most microbiological media producing either a white, pink (pyrimine) or red (prodigiosin) colony pigment production occurs between 12oc and 36oc. Biology 163 laboratory prodigiosin production in serratia marcescens (reviewed fall 2013) is a deep red pigment found within the bacterium serratia marcescens and causes the wild-type colonies to exhibit a deep red color lab research study. Lab results, and/or date of diagnosis reporter’s name 13 patient gender patient address report lab test results assure accuracy of testing performed following accepted procedures serratia marcescens – infection preventionists escherichia coli (e coli), shiga-toxin. Microbiology unknown project - 2014 instructions you should describe what tests you did, and describe/interpret any results you observed i will be collecting the lab notebooks at the end of the semester serratia marcescens shigella flexneri sporosarcina ureae staphylococcus aureus.